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p. 162. A spectrum of microscopieslocked

  • Terence Allen


In order to enable the eye to see detail beyond its normal range, we must provide it with a magnified image, illuminated in some way so we can actually visualize it, and with sufficient contrast to pick out separate parts within that image. The detail we can see is ultimately limited by the resolution of the imaging system we choose to use, but resolution without contrast is irrelevant. ‘A spectrum of microscopies’ considers the various parameters contributing to image formation across both light and electron microscopy—illumination, contrast, and resolution—and explains the units of microscopic measurement and the preparation of specimens.

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